Research progress of proteomics in bronchial asthmaThe first clinical medical college of Nanjing University of Chinese Medicine of Chinese m
Research progress of proteomics in bronchial asthma
The first clinical medical college of Nanjing University of Chinese Medicine of Chinese medicine Gao Junqing 09 class, 1 seven year zip code: 210023
Tutor: Yang Jibing First Clinical Medical College of Nanjing University of Chinese Medicine zip code: 210023
Abstract: proteomics, as a new field, has developed rapidly and made great progress. Protein as the bearer of life activities, has its special performance in the disease process, is of great significance for the diagnosis and treatment of some unknown pathogenesis of the disease, the recent progress of proteomics in bronchial asthma do a review.
Keywords: bronchial asthma proteomics
1 protein group theory and the study of bronchial asthma: asthma is a common disease, according to statistics the asthma patients about 300 million people, about 30 million of patients with asthma China, has seriously affected the people's physical and mental health. Its pathogenesis is not completely clear, is currently considered by mast cells, eosinophils, lymphocytes and other cells and cellular components involved in chronic airway inflammatory diseases of , which includes two aspects of host factors and environmental factors. In order to further study the pathogenesis of bronchial asthma, proteomics theory is applied to the study of bronchial asthma
2 the importance of proteomics research
2.1 proteome concept
Was first proposed by Australian scientist Marc Wilkins in 1994, all proteins in cells or tissues in different time and environment as the research object, to study the species, the protein from the overall interaction and function structure of knowledge . The overall analysis of protein expression can be carried out, and they can be identified and their functions in various tissues, organs or organisms are studied. .
The role of 2.2. in differential proteomics
Differential proteomics is an important strategy of proteomics research, focuses on the screening and identification of proteome differences and changes between samples of different species or conditions, has a strong realization of . It plays an important role in the selection of diagnostic markers, the study of the pathogenesis, the study of the mechanism of drug action, the monitoring of disease progression and the observation of curative effect.
Protein group has the characteristic of phase stability obviously, human disease status, role in the etiology of specific, lead to changes in physiology and pathology of the human body, the abnormal expression of proteins in a certain period of time and phase, can be expressed as the relative stability of .
The proteomics technology based and technology platform are: sample preparation, 1; 2, sample separation and analysis, including two-dimensional gel electrophoresis, two-dimensional chromatography, mass spectrometry, 3; quantitative proteomics; 4, antibody chip; 5, yeast two hybrid system; 6, protein phosphorylation group; 7, biosensor chip mass spectrometry and bioinformatics; 8.
2.3. proteomics and combination with traditional Chinese medicine:
In recent years, with the development and standardization of traditional Chinese medicine, proteomics has gradually entered the field of Chinese medicine diagnosis and treatment. The dynamic changes of the proteome can reflect the dynamic evolution of the body, and the micro differentiation of syndromes on the macro level is an important development and deepening of the overall concept of . The essence of syndrome is genotype and its expression. The changes of symptoms and signs in vitro can be observed by naked eye, which is the molecular basis of gene expression and the difference in mRNA and protein levels in vitro. . The concept of dynamic diversity, proteomic features and protein syndrome and complex relationship has in common with proteomics study of TCM syndrome essence of technology should be better able to explain the problem of  than at the genomic level.
Application of 3 proteomics in the pathogenesis of bronchial asthma:
Although the pathogenesis of asthma is not yet clear, but the theory of inflammation has been recognized by the medical profession, chronic airway inflammation persists. Ma Yong, ye  chapter through the investigation of 60 cases of bronchial asthma patients in different degree, found in healthy control group CC16 content reached 20.1ng/ml, and less than 5 years the mean content of CC16 is 7.38ng/ml, and more than 5 years group CC16 average content was 4.3ng/ml, that of CC16 protein gradually reduced detection is one of the clinical features of asthma when, which is worthy of attention. Roh  by two-dimensional electrophoresis and matrix assisted laser desorption / ionization time-of-flight mass spectrometry study of lung tissue in asthmatic mice, found that 36 proteins differentially expressed protein, microtubule -A, vesicular monoamine transporter, plasminogen and other related proteins may play a certain effect in the pathogenesis of allergic asthma in. Malmstrom  of patients with lung platelet-derived growth factor of Leydig cell activation pathway, determine the protein synthesis of PDGF affects cell remodeling process, demonstrated by PDGF in proteomics change has potential pathological effects on asthma; Luo Li, Li Ting, Cao Guiwen  24 mice were randomly divided into model group, asthma azithromycin treatment group and control group, the expression level was detected by immunohistochemistry in lung tissue of bronchial STAT5, STAT6, STAT5, the azithromycin treatment group STAT6 protein expression was significantly lower than that of asthma group (P< 0.01). Conclusion STAT5 and STAT6 are associated with the pathogenesis of asthma, and azithromycin can inhibit airway inflammation by down regulating the expression of STAT5 and STAT6.
4. The application of proteomics in the diagnosis of bronchial asthma:
Gao Xiaofang, Cheng Xiangduo, Hu glorious  by the determination of asthma patients before and after treatment with corticosteroids, and not treated asthma patients in the ECP group (serum eosinophil cationic protein) levels, serum ECP is that good asthma disease marker; Su Miao tours, Li Changchong, Luo Yunchun  study of 56 cases of children with asthma and platelet count (PLT), plateletcrit (PCT), mean platelet volume (MPV), platelet distribution width (PDW) and platelet large cell ratio (LPCR) of 5 parameters, we analyzed the serum eosinophil cationic protein (ECP) and the correlation between the levels of total IgE think, the level of serum ECP can reflect the clinical severity of asthma.
5. Research and application of proteomics in the treatment of bronchial asthma:
Xie Jiajia, Liang Jianhua, Peng Junjie and other  to the standard of 60 cases of patients with cough variant asthma with Xiaoqinglong Decoction and granule intervention for 4 weeks, that small Qinglong Decoction may clearly alleviate cough variant asthma by regulating IL-13 and NF- kappa B p65 expression; the original red, Dong Li, Zhang Yingmin and  in 60 cases of patients with mild to moderate asthma. Randomly divided into Tripterygium group, glucocorticoid group and control group before and after treatment, serum ECP FEV1, PEF and lung function were measured, the results of Tripterygium wilfordii can significantly reduce eosinophil cationic protein levels in serum of patients with asthma; Wan Lisheng, Zhu Jinshan, Wang Xialing and other  application of ovalbumin intraperitoneal injection and aerosol inhalation made the rat model of asthma, airway smooth muscle were observed with RT-PCR epithelial c- expression of fosmRNA, that Chuanfuling Babu agent may be inhibited through down-regulation of c- gene expression of FOS Rat airway smooth muscle cell proliferation to prevent asthma; Yan Xing Ke, Zhang Yan, Lin Pei Yu  40 SD rats were randomly divided into control group, saline control group, asthma model group, asthma acupuncture group, asthma model group 5 binding, detection of lung surfactant protein A (SP-A) expression. Prevention and treatment of allergic asthma that acupuncture can regulate the airway of asthmatic rats in SP-A.
6. The prospect of proteomics in the mechanism of bronchial asthma:
With the industrialization of society, bronchial asthma patients increased year by year, the prevention and treatment of asthma has aroused great concern in the community. In the use of the original diagnosis and treatment technology, we should develop new methods of diagnosis and treatment, to solve the problem of asthma pathogenesis. Proteomics, as a new type of life science and technology, has been highly valued. However, due to the TCM syndrome itself has certain uncertainty,
Proteomics technology also continues to improve , therefore, in the course of the study should be combined with the TCM syndrome characteristics, and continue to actively promote the progress of proteomics research, I believe that in the near future, the pathogenesis of asthma problem will be with the perfection and development of proteomics and smoothly done or easily solved.
1. Ma Lingfeng, Zhao Ying, and. The research progress of traditional Chinese medicine syndromes of bronchial asthma and the possibility of intervention of proteomics technology [J]. Chinese folk medicine.2005,25 (7): 86-87.
2. He He Ping, Zhao Heping. Proteomics research from genomics to [J]. biology bulletin, 2002, 37 (9): (in Chinese)
3, Espejel F, Roa JC.Surface enhanced laser
Desorption/ionization (SELDI): proteomics technology its application and in
Oncology[J].Med Clin, 2008131 (8): 312-317.
4. Zhao Hongwei, Tian Xiuzhu, and. The research and application of differential proteomics [J]. medicine and Philosophy (clinical decision forum edition).2006,4,27 (4): 45-47.
5. Meng Qinghong, Wang Changsong, et al. Feasibility study on the application of proteomics technology in TCM syndrome [J]. Journal of traditional Chinese medicine.2011,2,26 (2): 170-172. (in Chinese)
6, Yue Lifeng, Chen Jiaxu. Talk about the essence of TCM syndrome [J]. Jilin traditional Chinese medicine, 2007,27 (11): 1-2.
7, Chen Weijing, Liao Rongxin, Zhang Zheng. Analysis of TCM syndromes and proteomics [J]. modern medicine and health, 2007,23 (14): 2148-2149.
8. Song Jiannan. Proteomics study of TCM syndrome [Z].
9, Ma Yong, ye Zhang Fa. Analysis of CC16 protein in bronchial asthma patients with different course of disease [J]. Chinese Journal of practical medicine, 2008.7,3 (19): 73-74.
10, Robert son
TP, Dip P M, Ward JP, et al. of sustained hypoxic Inhibition vasoconstriction
By Y-27632 in intrapulmonary and perfused lung of the isolated arteries rat.Br
J Pharmacol, 2000, 131 (1): 5-9.
11, Malm st ROM
J, Tu fvesson E, Lofdahl CG, et al. of platelet-derived growth fact Activation or
Pathway in asthmatic pulmonary-derived human mesenchymal
Cells.Electrophoresis, 2003, 24 (1-2): 276-285.
12. Luo Li, Li Ting, and. The effects of azithromycin on the expression of STAT5 and STAT6 in lung tissue of asthmatic mice [J]. Chinese Journal of experimental diagnosis, 2010.10,14 (10): 1529-1531.
13, Gao Xiaofang, Cheng Xiangduo, Hu Guangrong. The serum eosinophil cationic protein determination and clinical significance of [J]. Journal of Henan University (Medical Science Edition), 2006.2,25 (1): 46-47.
14, Su Miao Miao, Li Changchong, Luo Yunchun. Study on the relationship between platelet parameters and eosinophil cationic protein in children with asthma [J]. Zhejiang Journal of clinical medicine, 2005.12,7 (12): 1247-1248.
15, Xie Jiajia, Liang Jianhua, et al. Based on IL-13, NF- kappa B p65 to explore the clinical effect of Xiao Qinglong Decoction on cough variant asthma [J]. Journal of traditional Chinese medicine (.2012.11.1,27) (1398-1400.) (in Chinese)
16, the original red, Dong Li, Zhang Yingmin et al. Effects of tripterygium glycosides on serum eosinophil cationic protein in asthmatic patients [J]. contemporary medicine, 2011.3,17 (8): 143-144.
17, Wan Lisheng, Zhu Jinshan, Wang Xialing et al. [J]. Journal of traditional Chinese medicine.2006.9,24 was deposited on airway epithelial cancer agent Babu Ling asthma rat c- gene expression of Fos (9): 1671-1672.
18, Yan Xingke, Zhang Yan, Lin Pei encyclical et al. Effect of the expression of SP-A [J]. China lotion with acupuncture on asthmatic rat model, 2010.8,30 (8): 665-668.
19. Ye Lei, Ouyang Xuenong, et al. Research progress of proteomics technology and its application in the field of traditional Chinese medicine [J]. Chinese cancer.2009,18 (3), 213-217. ()