The judgment of tumor recurrence were mainly tumor size and clinical symptoms, commonly used detection methods include imaging and pathologi
The judgment of tumor recurrence were mainly tumor size and clinical symptoms, commonly used detection methods include imaging and pathological method and other conventional methods, but these methods are lagging behind, not real-time monitoring, invasive and specific comparison etc.. It is a hotspot of current research to judge the curative effect, recurrence and prognosis of the tumor cells, which has the advantages of simple operation, real-time, non-invasive, flexible and so on.
Circulating tumor cells (circulating tumour cell, CTC) are tumor cells that are released into the peripheral blood circulation by spontaneous tumor or metastatic tumor. It was first discovered in 1869 by Asthworth, which is recognized as a marker for the detection of hematogenous metastasis of tumor. Some researchers believe that the number of CTC in the blood of patients with liver cancer is closely related to the development and prognosis of patients. Recently, Xu reported that the research team used the asialoglycoprotein receptor (asialoglycoprotein receptor) designed to separate the immunomagnetic beads CTC liver tumor, found in healthy volunteers, CTC was not detected in benign liver disease or non HCC patients in blood; and in 85 cases of hepatocellular carcinoma patients, 69 cases were detected CTC (positive rate 81%); each patient for 5 ml blood samples, detected the number of CTC is (19 + 24); the number of CTC infection and tumor positive rate and cell size, portal vein tumor thrombus formation and TNM stage were significantly related. Giovanna Vona et al. Through the separation of tumor cell size CTC showed that the CTC detected in the peripheral blood of patients with primary non metastatic liver cancer was significantly correlated with tumor proliferation and portal vein tumor thrombus. At the same time, there was a significant correlation between the number of circulating CTC and the number of patients with thrombosis, suggesting that the peripheral blood CTC has important clinical significance for cancer classification and prognosis. In addition, in the study of anti metastasis drugs, to determine the effect of anti metastasis drug can change in the number of CTC in the blood through the observation after treatment, and no need to spend time waiting to observe the change of metastatic lesions, can greatly shorten the research cycle.
As a new technique for the prediction of recurrence and metastasis of malignant tumors, CTC detection has the advantage that other detection techniques can not be compared. Not for biopsy, laparoscopy, invasive operation, 5 ml peripheral blood CTC detection can be completed only from patients, and without fasting; if in cancer surgery, radiotherapy and chemotherapy before and after dynamic detection of peripheral blood CTC number, to judge the prognosis of the patients, the disease free survival and overall survival has more clinical significance.