Detection of allergic disease refers to the detection of allergens that cause allergies. To find out whether the patient is allergic, allerg
Detection of allergic disease refers to the detection of allergens that cause allergies. To find out whether the patient is allergic, allergic to any allergen, the degree of allergy, for the guidance of patients targeted prevention and specificity of treatment are of great significance.
At present, allergen detection methods include two kinds of in vivo and in vitro assays. In vivo detection refers to a specific diagnostic method for a small amount of allergen purification into the body, or contact with skin, mucous membrane, and then observe the reaction. Including skin test, nasal mucosa, conjunctival test, etc.. At present, the most widely used skin test. The skin test also has several different methods, such as intradermal injection, prick, patch method. The principle is basically the same, one of the most widely used and reliable is the intradermal test method and its principle is prick test method, a small amount of allergens or injected into the skin prick allergy, if this is, after a period of time, the skin will appear the skin erythema, wheal, the more obvious that the more severe allergy. Because the prick method is intradermal test safer, less pain, patients are more likely to accept, so in recent years there is a trend to replace the intradermal prick.
In vitro detection mainly refers to the detection of allergic conditions by detecting the serum antibody sIgE. SIgE is also called the specific IgE, it is a special kind of allergic antibody contact allergic constitution in the patients after allergen produced, each allergen induced by sIgE are not the same, sIgE is arch-criminal causing allergic reaction. The higher the level of sIgE detected, the more species, the more severe the degree of allergy. Because allergic disease is caused by a series of reactions caused by IgE in vivo has led to the occurrence of a variety of allergic symptoms, so the detection of sIgE can better reflect the immune status of allergic reactions. In particular, it is very important to detect the degree of sIgE in vivo for desensitization, especially for desensitization. To observe the changes of sIgE in vivo after desensitization therapy is very important for the evaluation of curative effect and the formulation of late treatment. At present, there are many reagents in vitro, including qualitative and quantitative methods. Internationally recognized sIgE detection "gold standard" is the Swedish Pharmacia Co CAP allergen detection system.
In vivo detection of in vitro detection and comparison, detection in vivo by a number of factors is large, such as age, children, elderly skin reaction is not sensitive, so the test can reflect the degree of skin allergies is relatively low; the skin test was under the influence of drugs, such as skin test before taking anti allergy drug, if cold medicine, must stop drug a few days to skin test. However, because of the old age of skin test methods, the method is relatively mature, and the price is low, so it is still a sensitive and reliable diagnostic method.
Influencing factors of detection in vitro are relatively small, taking anti allergy drugs will not affect the test results, and the detection result is more objective and accurate, in vitro experiments in vivo is undoubtedly has more advantages, but because the reagents have to be imported, so the price is relatively expensive, we only look forward to the development of domestic reagents. To better the detection method is introduced.